Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

cDNA concentration of qPCR

cdna concentration qpcr

  • Please log in to reply
1 reply to this topic

#1 thinker

thinker

    member

  • Active Members
  • Pip
  • 16 posts
3
Neutral

Posted 20 March 2012 - 01:38 PM

Which the minor concentration of cDNA that can I use in qPCR?
And which would the best concentration?

#2 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,112 posts
59
Excellent

Posted 20 March 2012 - 05:53 PM

you need to look at the manual of your DNA polymerase. It must recommend a certain amount. The lowest could be 1 pg, but normally people use higher concentrations. we normally keep it below 1 ug because usually above that gives us smear on the gel.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.