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what is the best way to increase my purified enzyme's concentration???


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#1 mimiW

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Posted 19 March 2012 - 12:56 PM

what is the best way to increase my purified enzyme's concentration??? any protocol for concentrating enzymes? also best way to keep them alive n well for the next few month????


thanks!!!!

#2 phage434

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Posted 19 March 2012 - 04:31 PM

Probably the easiest is to use centrifugal filtration with an ultrafiltration membrane, as found in products such as the Millipore Centricon and Microcon systems. Dialysis against a solution high in PEG (or solid PEG) also will remove water but not the enzyme. Often, storage in 50% glycerol at -20C is a good approach for longer term.




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