what is the best way to increase my purified enzyme's concentration??? any protocol for concentrating enzymes? also best way to keep them alive n well for the next few month????
thanks!!!!
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#2
mdfenko
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Posted 20 March 2012 - 12:14 PM
you can concentrate by ultrafiltration, precipitation and resuspension, evaporation (lyophilization, speedvac, nitrogen stream).
there may be pitfalls to these methods (eg- co-concentration of medium components). you have to see which method will give you stoichiometric recoveries without denaturing the enzyme.
we generally prefer ultrafiltration (with a stabilizing agent when necessary) for final concentration of purified enzyme.
there may be pitfalls to these methods (eg- co-concentration of medium components). you have to see which method will give you stoichiometric recoveries without denaturing the enzyme.
we generally prefer ultrafiltration (with a stabilizing agent when necessary) for final concentration of purified enzyme.
Edited by mdfenko, 20 March 2012 - 12:15 PM.
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#3
Inmost sun
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Posted 03 April 2012 - 11:51 AM
think also of chromatography, I used Q columns to concentrate...
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Posted 15 May 2012 - 06:31 PM
is it true that by using fplc, you'll get more volume instead of hplc?
#5
mdfenko
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Posted 16 May 2012 - 07:03 AM
Sid, on 15 May 2012 - 06:31 PM, said:
is it true that by using fplc, you'll get more volume instead of hplc?
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
