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which protein can I use as a loading control for nuclear protein fraction?

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#1 memari

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Posted 19 March 2012 - 09:43 AM

which protein can I use as a loading control for nuclear protein fraction?
I have run several times and I have used Beta-Actin as control for nuclear protein fraction. but I have fluctuation between bands even after normalization by Biorad-Protein Assay.
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Babak Memari

#2 doxorubicin

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Posted 19 March 2012 - 01:14 PM

How about some histone proteins? There are some good Histone H3 antibodies. Also, you might consider Lamin A/C.

#3 Gangwolf

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Posted 28 March 2012 - 06:12 AM

We frequently use TATA binding protein for nuclear extracts as loading control. You can also check if you have cytosolic contamination in your nuclear fraction by probing for A/B tubulin, as tubulin should not be present in the nucleus.




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