Now I have a question.
My template is about 800 bp. I will want to use PCR product to do TA cloning.
When I use Taq polymerase for PCR, will the template of PCR reaction be added A ?
Because my friend said it that would be added A to the end of template sequence of PCR reaction.
If my friend is right.
I maybe will construct template to TA cloning.
Colud anyone can tell me?
Edited by yen0731, 19 March 2012 - 01:50 AM.