2 replies to this topic

[julnobody]

Hi all,

I have a question concerning phage infection during phage display for instance, how is it possible to know that only one phage is going to infect a bacteria during amplification ?


Is it possible to have bacteria infected with several phagemids(phage display vector) from M13 ? If not, how come superinfection with helper phage is possible?

Thats probably a really stupid question but I am just a beginner in the field ..

Thanks

[BioMiha]

If your multiplicity of infection (MOI) is low, you can be fairly certain according to the Poisson distribution that you will have only one phage per cell. If MOI is high then more phage can infect at once. Helper phage cannot replicate, but are added at a high MOI to ensure rescue of all displaying phage.

[julnobody]

BioMiha, on 19 March 2012 - 12:21 AM, said:

If your multiplicity of infection (MOI) is low, you can be fairly certain according to the Poisson distribution that you will have only one phage per cell. If MOI is high then more phage can infect at once. Helper phage cannot replicate, but are added at a high MOI to ensure rescue of all displaying phage.

Ok that makes sense, thank you very much !