Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Can we use ELISA capturing antibody for Western Blot Primary antibody?

Western Blot ELISA Primary antibody capturing antibody

  • Please log in to reply
1 reply to this topic

#1 It'sNotMe

It'sNotMe

    member

  • Active Members
  • Pip
  • 19 posts
0
Neutral

Posted 18 March 2012 - 05:44 AM

Hi,

Im very interested with this Ab: http://tools.invitro..._Rev%201008.pdf,

However, the application specified for ELISA...... I would like to use it for western blot. The supplier is doubtful on this. Is there any difference between ELISA capturing Ab and Western Blot Ab.

Thanks a lot!
It'sMe

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,758 posts
130
Excellent

Posted 20 March 2012 - 01:11 PM

elisa antibody will capture native protein.

the antibody for western blot binds to denatured protein.

sometimes the epitope is the same and the antibody can be used for both. sometimes it's not.

follow the advice of the supplier. the antibody would have been tested for various immuno-techniques.

Edited by mdfenko, 20 March 2012 - 01:11 PM.

talent does what it can
genius does what it must
i do what i get paid to do





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.