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blocking buffer containing BSA and cross reactivity

BSA specific non specific

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2 replies to this topic

#1 arachnide

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Posted 18 March 2012 - 03:23 AM

Could BSA present in the blocking buffer cross react with a mouse-anti human Ig detection antibody ? Thank you

#2 Curtis

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Posted 18 March 2012 - 08:29 PM

very unlikely, why?

#3 Ben Lomond

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Posted 28 March 2012 - 04:51 PM

Yes highly likely if the BSA is not IgG free and if the mouse anti-human IgG shows cross reactivity with bovine IgG.

There are a number of solutions to this problem.

Use IgG free BSA. Use an alternative non mammalian blocking solution. Use a mono detection regent with confirmed lack of cross reactivity with bovine IgG. Use a bovine serum protein cross absorbed polyclonal anti-human IgG reagent. Jackson, Bethyl, or Southern Bio are good vendors for these.





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