Could BSA present in the blocking buffer cross react with a mouse-anti human Ig detection antibody ? Thank you
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blocking buffer containing BSA and cross reactivity
Started by arachnide, Mar 18 2012 03:23 AM
BSA specific non specific
2 replies to this topic
#2
Curtis
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Posted 18 March 2012 - 08:29 PM
very unlikely, why?
#3
Ben Lomond
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Posted 28 March 2012 - 04:51 PM
Yes highly likely if the BSA is not IgG free and if the mouse anti-human IgG shows cross reactivity with bovine IgG.
There are a number of solutions to this problem.
Use IgG free BSA. Use an alternative non mammalian blocking solution. Use a mono detection regent with confirmed lack of cross reactivity with bovine IgG. Use a bovine serum protein cross absorbed polyclonal anti-human IgG reagent. Jackson, Bethyl, or Southern Bio are good vendors for these.
There are a number of solutions to this problem.
Use IgG free BSA. Use an alternative non mammalian blocking solution. Use a mono detection regent with confirmed lack of cross reactivity with bovine IgG. Use a bovine serum protein cross absorbed polyclonal anti-human IgG reagent. Jackson, Bethyl, or Southern Bio are good vendors for these.
Also tagged with one or more of these keywords: BSA, specific, non specific
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