Hi everyone,
I am facing a difficult challenge that will require your expertise. I am currently performing one-step RT-qPCR on a microfluidic system, where the droplets that are 0.5nL. Each droplet will consist of one cell. The calculation of total cell lysate will be equivalent to approximately 2000 cells/uL (2 cells/nL --> 2000 cells/uL). I need assistance in approaching this issue.
I have tried various titrations.
0
emulsion RT-PCR
Started by gtran89, Mar 17 2012 02:14 PM
emulsion microfluidics RT-PCR high cell lysates pcr inhibition
No replies to this topic
Also tagged with one or more of these keywords: emulsion, microfluidics, RT-PCR, high cell lysates, pcr inhibition
 |
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Primer Efficiency across runsStarted by Guest_Joe101_* , 08 May 2013  PCR, Primer, RT-PCR, Efficiency and 1 more...
|
|
|
|
|
|
Protocols and Techniques Forums →
siRNA, microRNA and RNAi →
Detecting miRNA mimics by RT-PCR?Started by Guest_SD1_* , 02 May 2013 miRNA, RT-PCR
|
|
|
|
Protocols and Techniques Forums →
Molecular Biology →
qPCR detectionStarted by Guest_wdk_* , 28 Feb 2013 qPCR, RT-PCR
|
|
|
||
Protocols and Techniques Forums →
PCR, RT-PCR and Real-Time PCR →
Why RNase H treatment after cDNA synthesisStarted by Guest_cellthetruth_* , 26 Feb 2013 RT-PCR, cDNA, RNase
|
|
|
||
Protocols and Techniques Forums →
Molecular Biology →
Best way to isolate viral mRNA for RT-PCR?Started by Guest_ACN_* , 25 Feb 2013 Isolating mRNA, mRNA, RT-PCR and 2 more...
|
|
|
