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RNA to spike


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#1 Chelo

Chelo

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Posted 17 March 2012 - 12:56 PM

Dear Forum members,

I am assessing the quality of different in-lab-made buffers to preserve RNA in my samples of interest. Since I only have tiny amounts of starting material, I am checking the preservation by quantitative RT-PCR. However, I found high variability in this process and I think this is because I cannot distinguish the preservation properties of the tested buffers from all the subsequent manipulation to real-time PCR. For instance, it could be the case that some of the salts in the buffers impede or facilitate the binding capacity of the columns I use to purify the RNA. So, I thought of adding some RNA to the treated sample just before the purification step. My question is whether there is any commercial source of RNA for this. Note that I should avoid human RNA and I would need around 1 ug per assay, so I need quite a lot of cheap RNA.
Thank you all in advance!




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