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strange bands shape agarose gel

small blebs agarose gel

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2 replies to this topic

#1 manicd

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Posted 17 March 2012 - 05:26 AM

Hello everyone.
I have a problem with my agarose gel electrophoresis.
The bands appear to be formed by small blebs.
Anyone Knows what the cause might be? Too much DNA per lane?
I have attached the picture of the agarose gel.
Thanks.

Michael

Attached Thumbnails

  • agarose gel.jpg


#2 Sotirios

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Posted 17 March 2012 - 03:08 PM

First check your gel coagulation. Maybe gel is soft and there is a problem with running bands. There is too much DNA.
Athanasiou Sotirios

#3 uzhamd

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Posted 18 March 2012 - 03:24 AM

there very large amount of plasmid DNA present. If you dilute the sample and then run the gel (1%) again u wont this prob hopefully.





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