Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Reuse of SDS-PAGE electrode buffers


  • Please log in to reply
2 replies to this topic

#1 Glyoxime

Glyoxime

    member

  • Active Members
  • Pip
  • 13 posts
2
Neutral

Posted 15 March 2012 - 12:26 PM

Hi everyone:

I have always heard that the anode buffer (outer chamber in Miniprotean 3) can be reused many times but the cathode buffer (inner chamber) must always be fresh. Is this true? If so, why?

Thanks!!

#2 Curtis

Curtis

    Metaller Scientist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,112 posts
58
Excellent

Posted 15 March 2012 - 07:38 PM

Biorad's? I always use just one tank buffer for Mini Protean 3 and re-use multiple times until it turns cloudy.

#3 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,704 posts
123
Excellent

Posted 16 March 2012 - 11:44 AM

because the cathode buffer will be depleted.
talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.