Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Quantification of LDL Oxidation

LDL oxidation lipoprotein electrophoresis TBARS

  • Please log in to reply
1 reply to this topic

#1 billtownsend1

billtownsend1

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 15 March 2012 - 04:03 AM

Hello all,

What are your thoughts on the best way in terms of cost/ease of use to quantify LDL oxidation after treatment with copper sulphate?

I've seen the TBARS assay and the use of gel electrophoresis (where the mobility of the oxidised LDL is compared to that of native LDL), but am unsure which is best and why.

Thanks,

Bill T

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,742 posts
127
Excellent

Posted 16 March 2012 - 11:34 AM

we watched the oxidation of ldl by copper sulfate on a spectrophotometer at 234nm (from this lancet paper).

rather than take periodic readings, we followed the reaction (see our paper: Malik, M.N., M.D. Fenko, A.M. Sheikh and H.M. Wisniewski, “Isolation of a-Tocopherol (Vitamin E) from Garlic”, J. Agric. Food Chem., 45, 817-819 (1997)). we were confirming the presence of vitamin e by inhibiting the oxidation of ldl.

Edited by mdfenko, 16 March 2012 - 11:38 AM.

talent does what it can
genius does what it must
i do what i get paid to do





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.