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Restriction buffer precipitates


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#1 brand

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Posted 15 March 2012 - 03:00 AM

Dears,

I just spotted that the restriction buffer from New England seems to have precipitates once its thawed out from -20. They are purchased at least before an year ago.Although the digestion is happening i just wondered is it a symbol to throw them away or is it a normal buffer characteristic.

#2 phage434

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Posted 15 March 2012 - 05:27 AM

The only precipitate common in these buffers is in T4 DNA ligase buffer, which must be resuspended carefully on thawing. I have not seen precipitates in other buffers.

#3 Curtis

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Posted 16 March 2012 - 07:23 PM

is it from the new line of High Fidelity REs?

#4 brand

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Posted 17 March 2012 - 11:07 AM

No dear,

its the NE buffer 2




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