I am having problems with the qPCR. In some of the wells, the ROX signal increases with the cycles and we are having strainge results for SybrGreen. We think it's a case of evaporation in the wells, but anyway, I don't understand wy the ROX signal increases, It it a total fluorescence what is measured and even the concentration of ROX increased with evaporation, the total amount of ROX would be the same. Can someone explain me that?
Increase in ROX signal
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