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Sterile Water (sWFI) as Negative Control for Sterility

water swfi negative control sterility

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#1 kamocasg

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Posted 14 March 2012 - 05:48 AM

I am currently evaluating the use of sterile water for injection (sWFI) as a negative control for Sterility testing. I am trying to find arguments as to why NOT to use water since in some instances, if use as a positive control, bacteria will not use, but I cannot find data supporting this.
Why use water instead of the media that is currently use to test samples?

Please I need advise as where to get information or any feedback you might have.

Thanks.

#2 GaoYuQing

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Posted 05 April 2012 - 10:41 AM

I don't see any real reason not to use water for negative control, as long as it's sterile. WFI isn't all that kind to bacteria, osmotically. But definately preferred would be the same media you're using for the rest of the testing if its sterile just so you eliminate one more factor.

#3 pito

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Posted 05 April 2012 - 10:56 AM

Injection in what?

It can have an influence.. so it all depends on whether it an influence your animals or whatever you are going to inject.

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.






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