Posted 14 March 2012 - 05:45 AM
I have cloned it in pGEX-6p1 and pET 28a. and expressed it but the expressed protein afer sonication when ran on SDS-PAGE is going in the pellet. so it seems that my protein is forming inclusion bodies. further I want to crystallize the protein. so I do not want to compromise with the concentration and structure of the protein. Please suggest me some cheap methods. ( the size of gene of interst is 966bp).
Posted 14 March 2012 - 10:07 AM
Posted 14 March 2012 - 10:27 AM
Posted 25 April 2012 - 04:31 PM