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inquiry about the methods for gene knockout in bacteria

gene knockout wild type bacteria method

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#1 joy123

joy123

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Posted 13 March 2012 - 01:28 PM

Hi I want to knockout a gene in a bacteria.

I am a new starter. Would you please give a general picture about how many methods I can choose, so that I can read more about them and choose a suitable one? I get confused when I read papers for their different methods, so I want to get a big picture first.

Thanks a lot! And look forward for your response!

#2 Inbox

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Posted 02 August 2012 - 03:13 AM

Its just flanking region of gene you have to choose. goal is to add resistant marker replacing middle portion of flank, then later removing resistant marker too.
- thats all you have mutant.





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