Hi I want to knockout a gene in a bacteria.
I am a new starter. Would you please give a general picture about how many methods I can choose, so that I can read more about them and choose a suitable one? I get confused when I read papers for their different methods, so I want to get a big picture first.
Thanks a lot! And look forward for your response!
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inquiry about the methods for gene knockout in bacteria
Started by joy123, Mar 13 2012 01:28 PM
gene knockout wild type bacteria method
1 reply to this topic
#2
prabhubct
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Posted 02 August 2012 - 03:13 AM
Its just flanking region of gene you have to choose. goal is to add resistant marker replacing middle portion of flank, then later removing resistant marker too.
- thats all you have mutant.
- thats all you have mutant.
“Those who mind don't matter, and those who matter don't mind.”
-- Bernard M. Baruch
-- Bernard M. Baruch
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