hi guys
A typical TaqMan protocol contains a denaturation step followed by a combined annealing and extension step at 55–60°C, instead of the traditional three-step PCR cycle of denaturation, annealing, and extension. my question is, does combined temperature affects Taq polymerase efficiency? and which Taq polymerase we use for Taqman Real Time assay?
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#2
Trof
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Posted 12 March 2012 - 04:41 AM
Is it a homework question?
What types of Taq polymerase do you know if you're saying "which Taq"?
What types of Taq polymerase do you know if you're saying "which Taq"?
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#3
tea-test
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Posted 12 March 2012 - 04:59 AM
At least, it should have a 5' to 3' exonuclease activity for TaqMan assays. 
The lower temperature will affect the TaqPol, the velocity of extension will be decreased (this is one reason why you should keep real-time PCR products short).
The lower temperature will affect the TaqPol, the velocity of extension will be decreased (this is one reason why you should keep real-time PCR products short).
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#4
Mehdi
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Posted 12 March 2012 - 09:07 AM
tea-test, on 12 March 2012 - 04:59 AM, said:
At least, it should have a 5' to 3' exonuclease activity for TaqMan assays.
The lower temperature will affect the TaqPol, the velocity of extension will be decreased (this is one reason why you should keep real-time PCR products short).
The lower temperature will affect the TaqPol, the velocity of extension will be decreased (this is one reason why you should keep real-time PCR products short).
Thank you so much. your post answered my question.
Edited by Mehdi, 12 March 2012 - 09:08 AM.
