i had purchase invitrogen caspase-3 colorimetrik kit for measure apoptosis in c57bl6 mice retina and substantia nigra tissues.when i read the datasheet of kit i realize that,kit was for just cell counting.
i have to make this kit with tissue.i asked this problem with invitrogen tech support but the could not help me.
at datasheet writing at assay procedure 'count cells and pellet 3-5x10^6 cells per sample'
i had searched literature bu i cant find anything about my situation
is the anyone use this kit or another brand?
how will i calculate SN and retina volumes.
shortly how will i do this kit?
thanks for help