Hi folks!
My experimental setup is that I want to check expression levels of a given gene in (gram-negative) bacteria under given stress situations (low pH, high temp, etc...). Therefor I'm trying to get a semiquantitative RT-PCR (maybe followed by a Nothern) started and I'm wondering if anybody here knows of a gene to take as control, i.e. a bacterial gene that is transcribed equally under different stimuli. I thought about 16S rRNA. Is this valid? Any Sugesstions... ?
Mike
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#1
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Veteran
Posted 27 May 2003 - 07:21 AM
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#2
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Enthusiast
Posted 29 May 2003 - 04:00 PM
I don't know much about bacteria. In human, there is evidence that some stimuli may also alter expression of house keeping genes. I have encountered this too. 18s rRNA is better than house-keeping genes. Please have a look at this paper http://www.ncbi.nlm.nih.gov/entrez/query.f...3&dopt=Abstract
Hula
Hula
