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star activity about BamH I and EcoR I


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#1 xjlei99

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Posted 26 May 2003 - 01:48 PM

hi, since both BamH I and EcoR I have the star activity, does this mean that it is very hard to get the clone if using these two enzymes?
thanks
john

#2 yossi.b

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Posted 29 May 2003 - 11:35 PM

hi
i did it once they have star activity but with special condition u can do it.
like u know 1 unit of enzyme cut 1 ug dna in one hour for dicrease star activity use less enzyme and less incubaton time do it in big volume (100 ul).

if it still with star activity write to my e-mail yossibuganim@yahoo.com
good luck yossi




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