Sequencing of PCR product
Posted 08 March 2012 - 01:20 AM
Posted 08 March 2012 - 07:47 AM
Also, I'm pretty sure they do all of the purifying for you so you can skip the purification
(I've always just sent my per product untouched, just checked on a gel for size and approx concentration)
Posted 08 March 2012 - 11:54 AM
I would purify the PCR using a PCR clean up kit or ethanol precipitation if you have single band products.
Edited by bob1, 08 March 2012 - 11:55 AM.
Posted 08 March 2012 - 04:51 PM
Posted 10 March 2012 - 12:59 PM
Oops, yeah, me and my unreliable typing...
Bob1 really meant "if you add both the competing reactions will give an an UNusable signal."