Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

homologous recombination between plasmid and plasmid

plsmid

  • Please log in to reply
No replies to this topic

#1 summersnows

summersnows

    member

  • Members
  • Pip
  • 3 posts
0
Neutral

Posted 08 March 2012 - 12:31 AM

hi!

lately, i found(predicted?) nov. gene in plasmid from Soil bacteria(sphingomonas sp.)

first of all, i want isolation that gene, but i was not working in e.coli(still trying!).

and i had to single point mutation of that gene

but, due to the plasmid size(it is 500kb!!), i can not control that anymore.

So, i want get some information about homologous recombination between plasmid and plasmid to control that gene.

if you have any experience, please let it know

or, anyone have some idea of contol that gene

thank you

Dong-uk kim




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.