Posted 07 March 2012 - 12:10 PM
I use Beta actin as house keeping gene in my Reverse transcription PCR experiments. It doesn't have a uniform pattern expression. share youre ideas.
Posted 07 March 2012 - 12:56 PM
Posted 08 March 2012 - 04:46 AM
and there: http://medgen.ugent....vdesomp/genorm/
But this applies only, if your genes are "randomly" controlled, not by a global down regulation of mRNA transcription like in adult stem cells.
Posted 08 March 2012 - 05:39 AM
De Jonge et al. furthermore tested a wide range of possible genes in order to use them as housekeeping genes and found quite a few more suitible ones like RPS13, RPL27, etc. I tested them for my experiments and they worked quite well!