I went the website of NEB to check what buffer did I use.
I found that use buffer 1 + BSA.
Now I have a question.
The activity of XhoI that use buffer 1 is 75,but that use buffer 4 is 100.
The activity of SacI that use buffer 4 is also 100.
Why does the handbook or website(http://www.neb.com/n...sp#.T1bpTIfxqYA)suggest to use buffer 1 for double digestion of XhoI & SacI ?
Why dose use buffer 4 to do that ?
Can I use buffer 4 to do that ?
Edited by yen0731, 06 March 2012 - 08:54 PM.