2 replies to this topic

[Divya Pothula]

Hi Everyone,

I am working on some eukaryote like proteins in sponges. I am working on an experimental basis to express some eukaryote like proteins...I have been using Arabinose induction...but recently heard that IPTG has a better protein expression.
Can anyone may be explain on a comparative basis for a better protein expression--whether IPTG or Arabinose, which is better and why?

Thanks

[pDNA]

You are going to express proteins in E. coli?

IPTG induced expression systems (like pET or lac) give you stronger expression but also higher basal expression levels in uninduced state (can be a problem for toxic proteins) ...arabinose induced expression systems (like pBAD) give you lower expression but with a more stringent control of basal expression (nearly zero). For both systems you need E. coli strains with a special genotype (DE3 strains for pET and araD strains for pBAD (e.g. TOP10) ...so you'll have to think about this as well.

Maybe if you could give us some more details?

Regards,
p

[Divya Pothula]

Thanks a lot P,

The proteins to be expressed are not toxic, they have an effect on the phagocytosis. So i have to make clones with ecoli and better understand their expression and their role. So was wondering which is the best method to express proteins and eventually prot-prot expression.

But, I will still need some strong proof to actually understand this, perhaps a paper/ article/ citations?

Thanks again
D