I co-transfect my cells with my shRNA and the target plasmids by the ration of 0.25:1, 0.5:1, 1:1 and 2:1. and i find the lower shRNA: target ration the better inhibit efficiency. How to explain this? Thank you.
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How to explain this result?
Started by yqluuf, Mar 06 2012 01:30 PM
1 reply to this topic
#2
pcrman
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Epigenetist
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Excellent
Excellent
Posted 11 April 2012 - 07:43 PM
Probably too much shRNA expression has a negative impact on small RNA function by saturating the small RNA processing pathway and Ago proteins.
