I have a plasmid with a short hairpin-RNA sequence in a pRS vector. I want to amplify this vector in bacteria but it is not amplifying whatsoever. It is ampicillin resistance. I am using GC10 E.Coli from Sigma and LB broth. I have read here about using low temperature growth cycle and chloramphenicol. Can anyone suggest the best way to improve yield of this plasmid?
Thanks in advance,
Amplification of shRNA sequence containing plasmid
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