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[alexinlab2]

Dear All,
    I have a plasmid with a short hairpin-RNA sequence in a pRS vector. I want to amplify this vector in bacteria but it is not amplifying whatsoever. It is ampicillin resistance. I am using GC10 E.Coli from Sigma and LB broth. I have read here about using low temperature growth cycle and chloramphenicol. Can anyone suggest the best way to improve yield of this plasmid?

Thanks in advance,

Sincerely,
Alex...