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Amplification of shRNA sequence containing plasmid


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#1 alexinlab2

alexinlab2

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Posted 05 March 2012 - 03:53 PM

Dear All,
I have a plasmid with a short hairpin-RNA sequence in a pRS vector. I want to amplify this vector in bacteria but it is not amplifying whatsoever. It is ampicillin resistance. I am using GC10 E.Coli from Sigma and LB broth. I have read here about using low temperature growth cycle and chloramphenicol. Can anyone suggest the best way to improve yield of this plasmid?

Thanks in advance,

Sincerely,
Alex...




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