I am running a 1% agarose gel, using Hyperladder 1 (bioline). 100V for about 45 mins. Also tried 80V and 90V. It was working and resolving fine but now its not. It looks like a big smudge and my bands are running like a 'w'. I am not doing anything differently from when it worked before, although the TBE buffer is a new batch. Could it be that?
It doesnt really matter as I know where my bands should be, but I need a good pic!
Thanks
