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troubleshooting sequencing results

uploading ABI runs

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7 replies to this topic

#1 nightingale

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Posted 03 March 2012 - 11:40 AM

Dear All,

how to upload my sequencing runs ???

a msg saying that am not permitted to upload this kind of file pops up Posted Image

any suggestions ???

always grateful,
nightingale
" The more you learn, the more you realize how little you know ... "

#2 bob1

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Posted 04 March 2012 - 11:51 AM

You'll only be able to attach a few common picture files (jpg, png, tif(?), bmp) and text, pdf, and maybe MS office files. You may be able to take a screenshot (print screen/prt sc button on a PC, or use Grab on a mac) if you can open your files in a multiline view.

Do you have a description of the problem?

#3 nightingale

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Posted 04 March 2012 - 12:12 PM

thanks bob1 for ur passing :)

actually i don't really know what's the problem ...

the sequencer read many Ns ... :( and this is the first time i encounter such a problem!
even upon repeating the sample ... it also failed ...

there is no problem with my purification, i checked the (gel pure product) on the gel and it gave me a beautiful band ...
" The more you learn, the more you realize how little you know ... "

#4 Trof

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Posted 04 March 2012 - 01:30 PM

It reads many Ns why? Is the sequence underneath "ugly" or fine? At least image would be fine. Can you display raw data in your sequence software?

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon


#5 nightingale

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Posted 06 March 2012 - 03:26 PM

i don't know :( althought the peaks are some what resolved ...
it is fine ... image you mean, i capture the chromatogram ???
you mean the .abi data ??
if yes, this is what am trying to attach here ...

grateful ... :)
" The more you learn, the more you realize how little you know ... "

#6 Trof

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Posted 07 March 2012 - 03:35 AM

I tried this: rename .ab1 file to .pdf and upload with Advanced uploder. That allowed the upload for me. We can download and rename it back.

By image I meant a picture, screenshot of the sequence. If there's nothing else.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon


#7 nightingale

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Posted 11 March 2012 - 05:43 AM

thanks Trof ... overwhelmed! Posted Image

am trying to do so ... but the .pdf file doesn't open anymore ... it says that the file is damaged..
i know i have bothered you enough with this ...
thanks alot ...
" The more you learn, the more you realize how little you know ... "

#8 Trof

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Posted 12 March 2012 - 06:47 AM

Of course it won't open. You need to rename it back before opening, to ab1.

See this file?
Attached File  test.pdf   153.64KB   114 downloads
I uploaded it, it looks like pdf but it's not, it's a sequence. Download, save and rename to test.ab1 and it opens as a sequence. Works for me.

Just upload the sequence renamed to pdf, leave opening to us Posted Image

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

'Normal' is a dryer setting. - Elizabeth Moon





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