Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

0

troubleshooting sequencing results

Started by nightingale, Mar 03 2012 11:40 AM

uploading ABI runs

Please log in to reply

7 replies to this topic

#1 nightingale

Veteran

Active Members
180 posts

3
Neutral

Posted 03 March 2012 - 11:40 AM

Dear All,

how to upload my sequencing runs ???

a msg saying that am not permitted to upload this kind of file pops up Posted Image

any suggestions ???

always grateful,
nightingale

" The more you learn, the more you realize how little you know ... "

Back to top

#2 bob1

Thelymitra pulchella

Global Moderators
4,337 posts

222
Excellent

Posted 04 March 2012 - 11:51 AM

You'll only be able to attach a few common picture files (jpg, png, tif(?), bmp) and text, pdf, and maybe MS office files. You may be able to take a screenshot (print screen/prt sc button on a PC, or use Grab on a mac) if you can open your files in a multiline view.

Do you have a description of the problem?

Back to top

#3 nightingale

Veteran

Active Members
180 posts

3
Neutral

Posted 04 March 2012 - 12:12 PM

thanks bob1 for ur passing

actually i don't really know what's the problem ...

the sequencer read many Ns ...

and this is the first time i encounter such a problem!
even upon repeating the sample ... it also failed ...

there is no problem with my purification, i checked the (gel pure product) on the gel and it gave me a beautiful band ...

" The more you learn, the more you realize how little you know ... "

Back to top

#4 Trof

Brain on a stick

Global Moderators
941 posts

66
Excellent

Posted 04 March 2012 - 01:30 PM

It reads many Ns why? Is the sequence underneath "ugly" or fine? At least image would be fine. Can you display raw data in your sequence software?

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

Back to top

#5 nightingale

Veteran

Active Members
180 posts

3
Neutral

Posted 06 March 2012 - 03:26 PM

i don't know

althought the peaks are some what resolved ...
it is fine ... image you mean, i capture the chromatogram ???
you mean the .abi data ??
if yes, this is what am trying to attach here ...

grateful ...

" The more you learn, the more you realize how little you know ... "

Back to top

#6 Trof

Brain on a stick

Global Moderators
941 posts

66
Excellent

Posted 07 March 2012 - 03:35 AM

I tried this: rename .ab1 file to .pdf and upload with Advanced uploder. That allowed the upload for me. We can download and rename it back.

By image I meant a picture, screenshot of the sequence. If there's nothing else.

Our country has a serious deficiency in lighthouses. I assume the main reason is that we have no sea.

I never trust anything that can't be doubted.

Back to top

#7 nightingale

Veteran

Active Members
180 posts

3
Neutral

Posted 11 March 2012 - 05:43 AM

thanks Trof ... overwhelmed! Posted Image

am trying to do so ... but the .pdf file doesn't open anymore ... it says that the file is damaged..
i know i have bothered you enough with this ...
thanks alot ...

" The more you learn, the more you realize how little you know ... "

Back to top

#8 Trof

Brain on a stick

Global Moderators
941 posts

66
Excellent

Posted 12 March 2012 - 06:47 AM

Of course it won't open. You need to rename it back before opening, to ab1.

See this file?

test.pdf 153.64K 74 downloads
I uploaded it, it looks like pdf but it's not, it's a sequence. Download, save and rename to test.ab1 and it opens as a sequence. Works for me.

Just upload the sequence renamed to pdf, leave opening to us Posted Image