Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

How can we design a primer ? step by step method

primer design principle step by step

  • Please log in to reply
4 replies to this topic

#1 srimadh

srimadh

    member

  • Active Members
  • Pip
  • 18 posts
3
Neutral

Posted 03 March 2012 - 01:10 AM

Dear Members,

Can any one tell me step by step process of Primer Designing ...

not by software but using ncbi databases manually.

Please help me through this ...

Edited by srimadh, 03 March 2012 - 01:19 AM.


#2 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,267 posts
76
Excellent

Posted 03 March 2012 - 09:47 AM

What do you mean by "using ncbi database" ?
Do you mean using this: http://www.ncbi.nlm....s/primer-blast/ ?


Have you checked this: http://www.premierbi...mer_Design.html (its a general page on how to design primers)
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#3 srimadh

srimadh

    member

  • Active Members
  • Pip
  • 18 posts
3
Neutral

Posted 06 March 2012 - 08:23 AM

Thanks Mr. Veteran,

The link that you had given about the primer design part was very useful.

and I have a doubt ... suppose if we need to design primers for a tetra arms pcr ...

then how to do ... i read some of the websites but couldnt get it .... can you please elaborate on this ...

thanks a lot for your support ...

#4 pito

pito

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,267 posts
76
Excellent

Posted 06 March 2012 - 09:45 AM

check: http://www.ncbi.nlm....icles/PMC55900/ and http://www.niab.com/...ARMS_barley.pdf
and here you can start desinging primers:
http://cedar.genetic...ml/primer1.html and http://cedar.genetic...source_sequence

I cant really help you with this, I am not really familiar with tetra arms pcr.
Maybe you could also start a new post with tetra arms pcr as title (because some people might not read this topic because its about general primer design)
If you don't know it, then ask it! Better to ask and look foolish to some then not ask and stay stupid.

#5 hobglobin

hobglobin

    Growing old is mandatory, growing up is optional...

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,508 posts
94
Excellent

Posted 06 March 2012 - 10:33 AM

you can try out this program for tetra arms pcr:
http://cedar.genetic...ml/primer1.html

The software is described here
One must presume that long and short arguments contribute to the same end. - Epicurus
...except casandra's that belong to the funniest, most interesting and imaginative (or over-imaginative?) ones, I suppose.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.