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#1 Oscar

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Posted 20 May 2003 - 07:17 AM

Dear all,
I would like to know if there will be any fluorscent if I transfact empty GFP vector into the cell? It's that a must to do some negative control during the GFP transfection experiment like transfect empty vector or no transfection? Or any other negative control should be included?

thanks very much

#2 mingyue

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Posted 06 July 2003 - 05:53 PM

Is there a promotor in front of the GFP coding sequence in the vector? Otherwise GFP will be be expressed.




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