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tissue handling protocol for different experiments needed

Hematoxylin and eos cryotome freezing tissue

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#1 Panda1772



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Posted 01 March 2012 - 07:07 AM

I would like to understand what is the process I should do for a tissue I took from a rat, after I take it, if I want to:
1) Freeze it for further later use, to keep cells and ECM intact
2) Prepare for cryotome
3) Stain with H and E without loosing the cells or ECM of natural tissue

Thank you so much in advance!!

Also tagged with one or more of these keywords: Hematoxylin and eos, cryotome, freezing tissue

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