I would like to understand what is the process I should do for a tissue I took from a rat, after I take it, if I want to:
1) Freeze it for further later use, to keep cells and ECM intact
2) Prepare for cryotome
3) Stain with H and E without loosing the cells or ECM of natural tissue
Thank you so much in advance!!
tissue handling protocol for different experiments neededHematoxylin and eos cryotome freezing tissue
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