Methionine for protein expression
Posted 29 February 2012 - 12:09 PM
I need information about this technique. Can tou advise me some articles on this theme, please?
Posted 29 February 2012 - 06:04 PM
The purpose of cloning vector is to obtain the desired target protein (amino acids) alone.
The N-terminal methionine (AUG) residue just initiates translation of your desired protein.
As you might know Initiation and termination codon are necessary for protein synthesis.
Hope this answers your question.
Posted 05 March 2012 - 01:28 AM