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Methionine for protein expression


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3 replies to this topic

#1 L u c a

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Posted 29 February 2012 - 12:09 PM

If Methionine coding sequence is inserted in front of the sequence of protein in cloning vector, it facilitates an expression of this protein.
I need information about this technique. Can tou advise me some articles on this theme, please?

#2 jarannihc

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Posted 29 February 2012 - 06:04 PM

Hi
The purpose of cloning vector is to obtain the desired target protein (amino acids) alone.
The N-terminal methionine (AUG) residue just initiates translation of your desired protein.
As you might know Initiation and termination codon are necessary for protein synthesis.
Hope this answers your question.

#3 L u c a

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Posted 01 March 2012 - 12:02 AM

jarannihc, actually no, sorry... I know this mechanism, I'd just like to read any article about this for writing my graduate work)

#4 Papaver

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Posted 05 March 2012 - 01:28 AM

Then you should do some literature research...that's common work for writing a thesis.




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