3 replies to this topic

[L u c a]

If Methionine coding sequence is inserted in front of the sequence of protein in cloning vector, it facilitates an expression of this protein.
I need information about this technique. Can tou advise me some articles on this theme, please?

[jarannihc]

Hi
The purpose of cloning vector is to obtain the desired target protein (amino acids) alone.
The N-terminal methionine (AUG) residue just initiates translation of your desired protein.
As you might know Initiation and termination codon are necessary for protein synthesis.
Hope this answers your question.

[L u c a]

jarannihc, actually no, sorry... I know this mechanism, I'd just like to read any article about this for writing my graduate work)

[Papaver]

Then you should do some literature research...that's common work for writing a thesis.