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Protein expression in yeast - Yeast Genotype

genotype yeast protein expression

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#1 jmanz

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Posted 29 February 2012 - 09:50 AM

I am trying to over express a protein in yeast and have been having some problems. The strain I am using has the following genotype:

MATalpha leu2 trp1 ura3-52 his3::GAL1-GAL4 pep4 prb1-1122

Using the genotype, how do I know if I need to suppliment any amino acids when expressing the protein? (I have been told I dont need to add any suppliments, but i'm not so sure about that... The manufactures instruction manual does not define this either).

Any help would be greatlt appreciated. Thanks.

#2 protolder

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Posted 29 February 2012 - 03:13 PM

Hola, as far as I know if you use to culture it Yeast Nitrogen Base, you need to add these aminoacids for that the strain is auxotrophic, but if you use YNB +casaminoacids or any peptone, you don´t need.But in the case or casaminoacids you have to add trytophan because they haven´t by the acid hidrolysis of casein to obtain them. Anyway check compositions of peptones if you use them. Buena suerte





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