3 replies to this topic

[dfranz]

Hi
I want to ask you what is the best anti-phospho ERK1/2 antibody for western blot. I ussually used one from Sigma (cataloge number: 9692), but it is just doesn´t working anymore.

Like I heed to all the steps of my protocol, I think i have a problem with the antibody, and that is why I would like to know what is the best antibody for detect activated diphosphorylated ERK1/2

PD: i work with mouse T cells lysates.

Thanks!

[doxorubicin]

The rabbit monoclonals from Cell Signaling are excellent.  Try one of these:

http://www.cellsigna...ducts/4370.html
http://www.cellsigna...ducts/4377.html
http://www.cellsigna...ducts/4376.html

They all indicate they detect dually phosphorylated ERK1/2 and also singly phosphorylated ERK1/2 (either the Thr or the Tyr depending on the antibody).  This was likely the case with your Sigma antibody as well.

[dfranz]

doxorubicin, on 29 February 2012 - 04:37 AM, said:

The rabbit monoclonals from Cell Signaling are excellent.  Try one of these:

http://www.cellsigna...ducts/4370.html
http://www.cellsigna...ducts/4377.html
http://www.cellsigna...ducts/4376.html

They all indicate they detect dually phosphorylated ERK1/2 and also singly phosphorylated ERK1/2 (either the Thr or the Tyr depending on the antibody).  This was likely the case with your Sigma antibody as well.

Thanks

[Gangwolf]

Try this one  http://www.scbt.com/...4-antibody.html
Way cheaper than Cell Signaling and it works great!