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Crosslinking at wrong energy level.

Started by aazmaish, Feb 28 2012 08:27 AM

Crosslinking

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#1 aazmaish

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Posted 28 February 2012 - 08:27 AM

Hi,
I am very new to protein studies. And i have a question.
I wrongly Crosslinked my cells at 300 mj/cm^2. I had to do that at 400mj/cm^2.Now i am so much worried that what could happen if crosslinking has been done on wrong energy level.
Can anyone please help me that what could be the bad effects (or good effects) of this mistake.?

Thanks in advance

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Crosslinking at wrong energy level.

#1 aazmaish

Also tagged with one or more of these keywords: Crosslinking

Should blocking step be included after cross-linking antibody to beads in immuno

Formaldehyde cross linking problem!!!

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