Hello, hope someone can give me some advice.
I did DNA/RNA soil extraction following Griffitts, 2000 method of extraction. Then I stored the extracted DNA/RNA in 1x TE buffer. My problem is that now that I want to digest the DNA to get only the RNA for RT-16SPCR it's impossible to digest completely the DNA. I read that there is a limited amount of DNA that can be digested in the DNase assay. To start with, my samples got a lot of DNA, they were stored in a buffer containing EDTA and I cannot modify the protocol for extraction because by now population in the soil has changed so I need to work with these extracts. Dilution is not an option because that would cause the lost of RNA which belongs to minority species in the soil.
I'd like to know if a part from the many kits that are in the market to purify RNA from gDNA there is a 'lab-made' way of doing so, taking in account the state of my extracts.
Thank you.
Monica
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