5 replies to this topic

[EAG]

I need to do IHC in 250 slides and I was wondering how much reagents per slide I need.

I need to considered how many microliters per slide?

Thanks!

(null)

[bob1]

It depends on the sample size (diameter in mm?)  I have seen tissue samples that are1-2 mm across, but also samples that fill the whole slide.  It will also depend on the techniques you plan on using and whether you are using an automated stainer or not.

[EAG]

The samples are small, something like 2-3mm and I will not use any automation

[Rsm]

I usually use 100ul for a whole slide (and that's too much), so I'd use 10ul for a small sample (5ul should work as well). Cover with parafilm to prevent drying.

[EAG]

Thanks!!

[ravibiosciences]

If you have time and your staining is not expensive . It would be good to optimize your conditions from 5-100microL, careful and dont get the slide completely dry. More the dye you use more the background

Good luck.