Hi All,
I'm currently undertaking a purification protocol that requires the use of fairly large amounts of benzonase as my protein is quite contaminated with nucleic acid. As benzonase is not the cheapest reagent I was wondering if anyone was aware of any generic alternative nucleases that might proivde a cheaper alternative.
Thanks for your help.
Iiyama
1 reply to this topic
#1
Posted 24 February 2012 - 01:01 PM
#2
Posted 28 February 2012 - 09:26 PM
A member of our lab simply treats her purified protin with DNAse to remove DNA.. Works fine for her and is pretty cheap
http://www.roche-app...6_14_6_1_3.html
http://www.roche-app...6_14_6_1_3.html
Also tagged with one or more of these keywords: Purification, Nuclease, Benzonase
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