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Alternatives to benzonase?

Purification Nuclease Benzonase

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#1 Iiyama

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Posted 24 February 2012 - 01:01 PM

Hi All,

I'm currently undertaking a purification protocol that requires the use of fairly large amounts of benzonase as my protein is quite contaminated with nucleic acid. As benzonase is not the cheapest reagent I was wondering if anyone was aware of any generic alternative nucleases that might proivde a cheaper alternative.

Thanks for your help.

Iiyama

#2 Bluefunk

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Posted 28 February 2012 - 09:26 PM

A member of our lab simply treats her purified protin with DNAse to remove DNA.. Works fine for her and is pretty cheap

http://www.roche-app...6_14_6_1_3.html





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