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Can we use denatured DNA standards on RNA formamide gels

Started by shuan, Feb 23 2012 04:48 PM
DNA RNA electrophoresis formamide northern blot

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#1 shuan

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Posted 23 February 2012 - 04:48 PM

Hi

Is it possible to use denatured DNA standards on RNA denaturing gels in order to evaluate the size of RNA. I've used denatured DNA in order to evaluate some known mRNA on northern blot and it gave correct size but I couldn't find any literature saying whether you could use DNA standards or otherwise. Hence, I would be much grateful if someone enlightens me regarding this topic.

Please find the attached copy of the blot.

Thanking you.
Shuan
Northern po.jpg

#2 bob1

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Posted 26 February 2012 - 11:41 AM

In theory it should work, though there might be slight charge differences that could change the apparent size of the product.

#3 mdfenko

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Excellent

Posted 27 February 2012 - 08:10 AM

and keep in mind that you are no longer looking at "base pairs", you are looking at "bases".
talent does what it can
genius does what it must
i do what i get paid to do
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