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Age of paraffin embedded tissue

Started by jjanisz, Feb 23 2012 12:39 AM

IHC protein archive material

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#1 jjanisz

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Posted 23 February 2012 - 12:39 AM

hi,
I wolud like to ask about acceptable age of paraffin embedded tissue for protein analysis by immunohistochemistry. How old can they be? Does immunohistochemistry work on archive material? We have some 5/6- year samples from HNSCC. We want to check occurence of gene products with altered expression.

I will be geratfull for an answer

joanna

#2 knuf

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Posted 23 February 2012 - 07:38 AM

We have done IHC on paraffin tissue as old as 9 years and had no issue.

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