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Can I do immunohistochemistry on this??

histochemistry heart mouse frozen

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#1 Kalyca

Kalyca

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Posted 22 February 2012 - 09:47 AM

Hi there,

I have some knockout mouse hearts that have been stored in the -80C freezer for some time. They were originally removed from the animal, retrogradely perfused to clear them of blood and then freeze clamped with Wollenberger clamps that were pre-cooled in liquid nitrogen. They were then stored in microfuge tubes in the -80C (probably for over 2 years now) and my PI is interested in doing some fluorescent immunohistochemistry on these hearts. Would this be possible? They are not embedded in OCT and were not frozen using isobutate.

Any advice would be greatly appreciated!

Kaly

#2 bob1

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Posted 22 February 2012 - 02:24 PM

It should be fine, so long as they were snap frozen.





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