Does anyone know if there are differences with using a flat-bottomed vs rounded-bottomed 96-well plate when measuring protein content in unknown lysates?
Would there be any accuracy differences between them and would anyone know the mechanism by which light goes through these plates for measurement purposes?
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Round/flat-bottomed 96-well plates for BCA chemiluminescence protein detection
Started by science noob, Feb 21 2012 10:59 PM
2 replies to this topic
#2
Curtis
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Metaller Scientist
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Posted 22 February 2012 - 04:07 AM
i used flat for Bradford method
#3
bob1
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Thelymitra pulchella
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Posted 22 February 2012 - 02:14 PM
Flat bottom is definitely better for absorbance as the round bottom causes scattering of the light, leading to erroneous readings.
