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Round/flat-bottomed 96-well plates for BCA chemiluminescence protein detection


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2 replies to this topic

#1 science noob

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Posted 21 February 2012 - 10:59 PM

Does anyone know if there are differences with using a flat-bottomed vs rounded-bottomed 96-well plate when measuring protein content in unknown lysates?

Would there be any accuracy differences between them and would anyone know the mechanism by which light goes through these plates for measurement purposes?

#2 Curtis

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Excellent

Posted 22 February 2012 - 04:07 AM

i used flat for Bradford method

#3 bob1

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Posted 22 February 2012 - 02:14 PM

Flat bottom is definitely better for absorbance as the round bottom causes scattering of the light, leading to erroneous readings.




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