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Has anyone ever had a large insert when subcloning? Mine is 4.3kb?


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#1 kathyoc

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Posted 21 February 2012 - 08:59 AM

Have been trying to clone this for 8 weeks.. the insert is 4.3kb and the vector is 5.3kb! No luck... :(

#2 phage434

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Posted 21 February 2012 - 11:24 AM

"This." What could you possibly expect us to know about "this." My sympathy is all I can offer.

#3 kathyoc

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Posted 21 February 2012 - 11:30 AM

oh dear... clone with a large insert (4.3kb).. and a vector which is 5.3kb...




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