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plasmid extraction

gel

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5 replies to this topic

#1 hidayahwannur

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Posted 21 February 2012 - 05:13 AM

all pictures are the same, which one look better? and does the result ok ?

210212 plasmid extraction.jpg 210212.jpg 210212 ok 2.jpg 210212 ok.jpg 210212 okk.jpg 210212 plasmid dayah.jpg 210212 plasmid extraction.jpg

#2 HOYAJM

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Posted 21 February 2012 - 09:43 AM

It could be your plasmid. We can not tell you definitively if it is your plasmid. We know nothing about the plasmid. What I can tell you is there can be 3 bands from a plasmid prep (nicked, linear, supercoiled). If those bands are in the vicinity of the correct size, that should be your plasmid. The ladder on the right is very faint, and the ladder to the left is not necessary.

#3 hidayahwannur

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Posted 21 February 2012 - 10:08 AM

It could be your plasmid. We can not tell you definitively if it is your plasmid. We know nothing about the plasmid. What I can tell you is there can be 3 bands from a plasmid prep (nicked, linear, supercoiled). If those bands are in the vicinity of the correct size, that should be your plasmid. The ladder on the right is very faint, and the ladder to the left is not necessary.


thank you.. but how about the gel? i need also your comment about the gel.. is there the band? can you explain to me about the gel..what else i can modify ?

#4 HOYAJM

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Posted 21 February 2012 - 11:01 AM

I think the gels are probably OK. It could be the photography that makes them tough to interpret. there are bands definately there. If they are the correct bands, well that is for you to determine.

#5 mdfenko

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Posted 21 February 2012 - 12:25 PM

half of the pictures have a strange background pattern. i assume they show up when increasing exposure to better see the faint bands. you should find a way to eliminate the background and reshoot the gel with the better of the long exposures.
talent does what it can
genius does what it must
i do what i get paid to do

#6 hidayahwannur

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Posted 21 February 2012 - 03:18 PM

thank you for all the supportive comment. i really appreciate it and i will do more to get the skill





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