pH of RIPA buffer??
Posted 21 February 2012 - 01:06 AM
I have prepared RIPA buffer with the following ingredients:
50mM TRIS, pH8
0.5% sod. deoxycholate
Immediately after buffer preparation I measured its pH and it was 8.35.
I would like to know should I re-adjust the pH back to 8?
one more thing, regarding storage, some members recommend that it should be at -20, but others say that as long as there is no protease or phosphatase inhibitor cocktails it can be stored at refregirator at 4, which is the best temperature for storage?
Posted 21 February 2012 - 03:12 AM
I always had an alternate hypothesis....
Posted 21 February 2012 - 12:16 PM
sds makes pH readings questionable (it precipitates in and blocks the porous plug on the electrode due to kcl).
genius does what it must
i do what i get paid to do